19 Aug Neuropathy Relief Center Miami Research Review: Effects of electromagnetic field (PEMF) exposure at different frequency and duration on the peripheral nerve regeneration: in vitro and in vivo study
“PEMF enhanced peripheral nerve regeneration, and that it may be due to cell proliferation and increase in BDNF and S100 gene expression.”
Purpose: The purpose was to clarify the influence of frequency and exposure time of pulsed electromagnetic fields (PEMF) on the peripheral nerve regeneration.
Materials and methods: Immortalized rat Schwann cells (iSCs) (1 × 10(2)/well) were exposed at four different conditions in 1 mT (50 Hz 1 h/d, 50 Hz 12 h/d, 150 Hz 1 h/d and 150 Hz 12h/d). Cell proliferation, mRNA expression of S100 and brain-derived neurotrophic factor (BDNF) were analyzed. Sprague-Dawley rats (200-250 g) were divided into six groups (n = 10 each): control, sham, 50 Hz 1 h/d, 50 Hz 12 h/d, 150 Hz 1 h/d and 150 Hz 12 Hr/d. Mental nerve was crush-injured and exposed at four different conditions in 1 mT (50 Hz 1 Hr/d, 50 Hz 12 Hr/d, 150 Hz 1 h/d and 150 Hz 12 h/d).
Nerve regeneration was evaluated with functional test, histomorphometry and retrograde labeling of trigeminal ganglion.
Results: iSCs proliferation with 50 Hz, 1 h/d was increased from fourth to seventh day; mRNA expression of S100 and BDNF was significantly increased at the same condition from first week to third week (p < .05 vs. control); difference score was increased at the second and third week, and gap score was increased at the third under 50 Hz 1 h PEMF compared with control while other conditions showed no statistical meaning.
Axon counts and retrograde labeled neurons were significantly increased under PEMF of four different conditions compared with control. Although there was no statistical difference, 50 Hz, 1 h PEMF showed highest regeneration ability than other conditions.
Conclusion: PEMF enhanced peripheral nerve regeneration, and that it may be due to cell proliferation and increase in BDNF and S100 gene expression.
If you have been diagnosed with peripheral neuropathy or have neuropathy symptoms such as pain, burning, numbness in your arms, hands, legs or feet. Please contact us at (305) 275-7474 to schedule and discuss your questions with our new patient specialist or message us your number/email and our team member will reach out to you for scheduling.
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Dr. Alfonso Neuropathy Treatment Protocol
● Improves and increases oxygen and nutrition through the increase of circulation to the damaged nerves.
● Repairs peripheral fibers by stimulating regeneration of new nerve cells.
● Removes and eliminates mechanical peripheral nerve entrapments and compressions in the spine and extremities.
Dr. Alfonso‘s Neuropathy Treatment Protocol utilizes 8 specialized treatments designed to improve the supply of oxygen, eliminate waste, and improve nutrition to damaged nerves.
Increased circulation to the damaged peripheral nerves combined with treatments to stimulate peripheral nerve cell regeneration, and repair.
For more info on the Dr. Alfonso Neuropathy Treatment Protocol please visit our treatment page of our website.
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Sincerely Yours for Health,
Dr. Rodolfo Alfonso, D.C.
8585 Sunset Drive,
Miami, FL 33143